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Desai – Effect of TRPV4 overexpression on TLR responses in murine lung epithelial E10 cells

 

Effect of TRPV4 overexpression on TLR responses in murine lung epithelial E10 cells (2024)

Undergraduate: Palak Desai


Faculty Advisor: Silvio Antoniak
Department: Pathology and Laboratory Medicine

The transient receptor potential cation channel subfamily V member 4 (TRPV4) is expressed in the lung by epithelial cells (EpC). TRPV4 is activated by heat, mechanical force, hypo-osmolarity, and arachidonic acid metabolites. TRPV4 has been described as a target for treating inflammatory disease and IAV infection. Toll-like receptors (TLRs) are expressed on resident cells like EpCs within the lung and when activated act as a defense against pathogens. With stimulation of signaling cascades, inflammatory cytokines are released. TLR7 recognizes single strand (ss) RNA from ssRNA virus genomes including from IAV. In addition, TLR7 can be activated by the compound R848. Double-stranded (ds) RNA is detected by TLR 3. In this study, we aimed to investigate the inflammatory response of the murine lung EpC line E10 to TLR 3, TLR 4, and TLR 7 stimulations. We also investigated the effect of TRPV4 overexpression on the TLR 3, TLR 4, and TLR 7 responses.