Understanding how elevated temperature impairs Cdc6G260D function in Saccharomyces cerevisiae (2013)

Undergraduate: Samuel Neal

Faculty Advisor: Ginnie Hench
Department: Biology

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Cdc6 is essential for proper origin licensing and this has been shown previously with a temperature sensitive mutant form of Cdc6 known as Cdc6G260D. It is temperature sensitive in that the protein is nonfunctional causing failed DNA replication and slowing cell growth at elevated temperatures.

No one has researched the cause of the loss of function of Cdc6G260D at elevated temperature. This was the goal for this summer.

Hypothesis: Cdc6G260D is being degraded at the higher temperatures.

Goal: Make a budding yeast (Saccharomyces cerevisiae) strain containing both Cdc6 and Cdc6G260D, then observe levels of both proteins over time at elevated temperature

Results: A construct was made, but it was not able to be detected.